As a control, Nop1p (fibrillarin), a nucleolar protein (Venema and , 1995), was visualized with the mouse monoclonal Ab (mAb) A66 and mouse Abs conjugated to Texas Red (TR). DNA was stained with 4,6diamidine2phenylindole (DAPI) to localize the nucleus. As shown in Figure 3A, treatment with both Rcl1p and Nop1p Abs showed a typical crescentshaped staining, characteristic of nucleolar proteins.
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